Quantitative analysis of nitrifying communities in short-cut nitrogen removal from municipal wastewater of SBR - 文章详情页

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To　investigate　the　effects　of　real-time　control　on　the　structure　of　nitrifying　communities　of　the　short-cut　nitrogen　removal　in　SBR　pilot-scale　plant　with　a　working　volume　of　54　m,　molecular　techniques　of　Fish,　PCR-DGGE　and　PCR-Cloning-Sequencing　were　used　to　analyze　ammonia-oxidizing　bacteria　(AOB)　and　nitrite-oxidizing　bacteria　(NOB)　with　the　performance　of　short-cut　nitrogen　removal.　Fish　results　show　that　in　short-cut　nitrogen　removal　process,　AOB　became　dominant　with　compared　to　NOB　and　accounted　for　3%-12%　of　the　total　biomass.　More　importantly,　no　NOB　was　detected.　PCR-DGGE　gel　results　show　that　AOB　were　phylogenetically　related　to　Nitrosomonas-like　species　with　a　melting　point　at　the　range　between　30%　to　50%.　PCR-Cloning-Sequencing　results　show　that　all　clones　were　affiliated　with　Nitrosomonas　corresponding　to　DGGE　results　and　60%　of　the　clones　were　affiliated　with　Nitrosomonas　europaea.

关键词：

Real time control Cloning Biomass Ammonia Wastewater Melting point Nitrogen removal Bacteria Denitrification

作者机构：

[ 1 ] [Yang, Qing]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
[ 2 ] [Peng, Yong-Zhen]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
[ 3 ] [Zeng, Wei]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
[ 4 ] [Yang, An-Ming]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
[ 5 ] [Satoh, Hiroyasu]Institute of Environmental Studies, University of Tokyo, Tokyo 113-8656, Japan
[ 6 ] [Mino, Takashi]Institute of Environmental Studies, University of Tokyo, Tokyo 113-8656, Japan

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