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作者:

Yang, Qing (Yang, Qing.) | Peng, Yong-Zhen (Peng, Yong-Zhen.) (学者:彭永臻) | Zeng, Wei (Zeng, Wei.) (学者:曾薇) | Yang, An-Ming (Yang, An-Ming.) | Satoh, Hiroyasu (Satoh, Hiroyasu.) | Mino, Takashi (Mino, Takashi.)

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摘要:

To investigate the effects of real-time control on the structure of nitrifying communities of the short-cut nitrogen removal in SBR pilot-scale plant with a working volume of 54 m, molecular techniques of Fish, PCR-DGGE and PCR-Cloning-Sequencing were used to analyze ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) with the performance of short-cut nitrogen removal. Fish results show that in short-cut nitrogen removal process, AOB became dominant with compared to NOB and accounted for 3%-12% of the total biomass. More importantly, no NOB was detected. PCR-DGGE gel results show that AOB were phylogenetically related to Nitrosomonas-like species with a melting point at the range between 30% to 50%. PCR-Cloning-Sequencing results show that all clones were affiliated with Nitrosomonas corresponding to DGGE results and 60% of the clones were affiliated with Nitrosomonas europaea.

关键词:

Ammonia Bacteria Biomass Cloning Denitrification Melting point Nitrogen removal Real time control Wastewater

作者机构:

  • [ 1 ] [Yang, Qing]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
  • [ 2 ] [Peng, Yong-Zhen]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
  • [ 3 ] [Zeng, Wei]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
  • [ 4 ] [Yang, An-Ming]Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China
  • [ 5 ] [Satoh, Hiroyasu]Institute of Environmental Studies, University of Tokyo, Tokyo 113-8656, Japan
  • [ 6 ] [Mino, Takashi]Institute of Environmental Studies, University of Tokyo, Tokyo 113-8656, Japan

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来源 :

Journal of Beijing University of Technology

ISSN: 0254-0037

年份: 2007

期: 8

卷: 33

页码: 843-848

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