The　influence　of　the　different　operating　conditions　(the　trans-membrane　pressure,　feed　concentration　and　temperature)　on　dead-end　MF　specific　resistance　of　yeast　suspensions　was　studied　by　using　a　self-making　microfiltration　cup　in　which　the　PES　flat　membrane　with　0.2　μm　average　pore　size　was　put.　In　addition,　a　linear　multi-regression　model　was　used　to　study　the　degree　of　the　influence　of　these　operating　conditions　on　dead-end　MF　specific　resistance　of　yeast　suspensions.　The　results　showed　that,　the　trans-menbrone　pressure,　feed　concentration　and　temperature　are　all　influence　factors　of　dead-end　MF　specific　resistance,　and　the　operating　pressure　is　a　remarkable　factor,　both　the　feed　concentration　and　temperature　are　common　influence　factors;　the　influence　degrees　of　the　trans-membrane　pressure,　feed　concentration　and　temperature　are　75.31%,　12.89%,　11.8%,　respectively;　the　specific　resistance　increases　as　the　increasing　trans-membrane　pressure,　increases　with　the　increasing　feed　concentration　first　and　then　it　decreases　as　concentration　increases,　whereas　decreases　as　temperature　increase;　a　relationship　between　specific　resistance　and　the　trans-membrane　pressure,　feed　concentration　and　temperature　is　described　by　the　linear　multi-regression　model.　The　average　error　between　experimental　value　and　model　value　is　less　than　5.16%.　These　will　provide　basis　for　process　optimization　in　dead-end　microfiltration.