PCR　micro-fluidic　biologic　chip　was　fabricated　on　PMMA　substrate　by　using　excimer　laser.　The　impact　of　excimer　laser＇s　fluence　and　the　stage　velocity　on　microchannel＇s　qualities　including　depth　and　roughness　was　analysed.　Smoother　microchannel　could　be　etched　by　using　lower　laser　fluence　and　stage　velocity.　The　whole　sealed　PMMA　based　on　PCR　micro-fluidic　biologic　chip　was　bonded　by　hotpress　method.