The　flat　sheet　PTFE　membrane　was　modified　into　hydrophilicity　via　deposition　of　fluorinated　cationic　surfactant.　The　anti-fouling　property　of　the　membranes　was　investigated　using　lysozyme　(LYS)　and　humic　acid　(HA)　as　typical　foulants　and　further　evaluated　by　Derjaguin-Landau-Verwey-Overbeek　(XDLVO)　theory.　The　results　indicated　that　the　wettability　of　the　PTFE　membrane　can　be　manipulated　by　concentration　of　surfactant　solution,　and　the　super　hydrophilic　membrane　with　water　contact　angle　of　2.1　degrees　was　obtained　with　0.8　wt%　of　surfactant　solution.　The　super　hydrophilic　PTFE　membrane　showed　highest　cleaning　efficiency　of　62.5%　and　better　anti-fouling　property.　XDLVO　analysis　further　suggested　that　the　super　hydrophilic　PTFE　membrane　had　the　strong　repulsion　force　to　HA　and　LYS　and　displayed　better　anti-fouling　property　due　to　the　highest　positive　total　interaction　energy　(Delta　G(TOT))　value.　Linearly　fitting　on　membrane　practical　fouling　process　and　XDLVO　results　proved　the　consistency　of　the　XDLVO　theory　with　the　practical　anti-fouling　process.