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Abstract:
Objective: As ultrasound has become an important therapeutic technique on tumor, in order to find the cell-killing effect of focused bi-frequency ultrasound activated hematoporphyrin derivatives, experimental study was processed in vitro. Methods: Tumor cells K562 and SW-480 were killed by focused bi-frequency ultrasound activated hematoporphyrin (Hp) derivatives under optimized ultrasonic parameters and the cell-killing effect was tested by MTT (Thiazolyl blue: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromide) technique. Results: The lower frequency (0.6 MHz) was better than the higher (1.1 MHz) in activating hematoporphyrin derivatives; the bi-frequency was better than monofrequency, and the cell-killing efficiency was 2-3 times of sum of the two kinds of ultrasounds which constitute bifrequency. Incubation for the 16 h was better than 4 h after the cells exposed to the ultrasound. Conclusion: The method of killing tumor cells by focused bi-frequency ultrasound activated hematoporphyrin derivatives is presented and evaluated in this paper. Copyright © 2006 ACPSEM/EA.
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Australasian Physical and Engineering Sciences in Medicine
ISSN: 0158-9938
Year: 2006
Issue: 3
Volume: 29
Page: 267-271
ESI Discipline: MOLECULAR BIOLOGY & GENETICS;
Cited Count:
SCOPUS Cited Count: 3
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 2
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