For　the　deviation　occasionally　occurred　in　traditional　anthrone-H2SO4　spectrophoto-metry　method　in　determination　of　the　polysaccharide　content　of　Lycium　barbarum,　a　proper　measurement　condition　is　proposed　in　order　to　improve　the　determination　accuracy:　625.0　nm　of　absorption　wavelength;　2.00　mL　of　polysaccharide　solution;　4.00　mL　of　anthrone-H2SO4　reagent;　10　minutes　of　hydrolysis　and　chromogenic　reaction　time　at　room　temperature.　The　test　result　shows　that　the　determination　accuracy　is　improved　by　the　modified　method,　with　average　recovery　rate　of　98.93%　(R=0.835%,　n=6).　It　also　can　be　references　for　other　kind　of　polysaccharides　mass　fraction　determination.