A　pilot-scale　nutrient　removal　activated　sludge　system,　based　on　the　A2O　configuration,　was　used　to　treat　brewery　wastewater.　In　the　experiment,　the　existence　of　denitrifying　phosphorus　removing　bacteria　(DPB)　and　the　contribution　of　DPB　to　phosphorus　removal　was　observed.　The　tests　showed　the　average　total　amount　of　anoxic　P　uptake　accounted　for　70%　of　the　total　amount　of　P　uptake　of　the　system　at　steady　state,　oxygen　requirement　could　be　decreased　significantly　(about　25%).　Denitrifying　phosphate　removal　resolved　the　competition　for　organic　substrates　between　Poly-P　organisms　and　denitrifiers　under　lower　organic　loading.　Effluent　total-P　concentration　could　achieve　less　than　0.30　mg·L-1　and　average　effluent　total-N　was　less　than　10　mg·L-1.　It　is　clearly　demonstrated　that　the　phosphorus　uptake　almost　stopped　in　an　over-aerated　system,　the　released　phosphorus　could　not　be　taken　up　fully　again.　Consequently,　incomplete　phosphorus　uptake　led　to　temporary　reduction　of　biological　phosphorous　removal　efficiency.　It　took　about　the　time　of　one　sludge　age　to　eliminate　that　disturbance.