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作者:

Xi, Chen (Xi, Chen.) | Yiwei, Wang (Yiwei, Wang.) | Weijie, Bao (Weijie, Bao.) | Can, Wang (Can, Wang.) | Zhihai, Wang (Zhihai, Wang.) | Jingang, Gui (Jingang, Gui.)

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摘要:

Sample deposition based on micro-droplet ejection has broad application prospects in the field of biomedicine. For biological cell printing, a hybrid pneumatic-electrohydrodynamic (HPEHD) micro-droplet ejection system is built in the laboratory. Strong electric field is established by applying a high voltage between the nozzle and a collector electrode. A solenoid valve is opened temporarily; high pressure gas enters the liquid storage chamber, and produces pressure pulse, which extrudes the liquid slightly from the nozzle. The liquid is further deformed in the electric field into a cone shape (Taylor cone), and then the end of the Taylor cone breaks to form a micro-droplet. The ejection process is studied using machine-vision and image processing. With Sodium Alginate (1.0%) containing human peripheral blood mononuclear cells (PBMC) as bioink, single droplet per ejection is realized, and the droplet size is reduced by 50% due to the presence of the electric field. Through flow cytometry and microscopic photos, the effect of ejection process on the short-Term viability of cells is shown to be negligible, suggesting HPEHD ejection a potential technology option for cell printing. © 2019 Association for Computing Machinery.

关键词:

Bioinformatics Drops Electrohydrodynamics Image processing Liquids Nozzles Petroleum prospecting Pneumatics Sodium alginate Solenoid valves

作者机构:

  • [ 1 ] [Xi, Chen]Beijing Institute of Pediatrics, Beijing Children's Hospital, Capital Medical University, Beijing; 100035, China
  • [ 2 ] [Yiwei, Wang]Faculty of Information Technology, Beijing University of Technology, Beijing; 100124, China
  • [ 3 ] [Weijie, Bao]Faculty of Information Technology, Beijing University of Technology, Beijing; 100124, China
  • [ 4 ] [Can, Wang]Laser Engineering Research Institute, Beijing University of Technology, Beijing; 100124, China
  • [ 5 ] [Zhihai, Wang]Faculty of Information Technology, Beijing University of Technology, Beijing; 100124, China
  • [ 6 ] [Jingang, Gui]Beijing Institute of Pediatrics, Beijing Children's Hospital, Capital Medical University, Beijing; 100035, China

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年份: 2019

页码: 130-134

语种: 英文

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