Microinjection　technology　has　become　a　common　means　of　injecting　foreign　materials　to　a　target　cell　using　a　sharp-tipped　glass　micropipette　under　a　microscope　in　medical　and　biological　related　research.　Applied　forces　and　injection　speed　have　significant　impacts　on　the　success　of　cell　injection.　This　paper　presents　the　mechanic　analysis　and　injection　velocity　optimization　of　cell　injection.　Firstly,　a　viscoelastic　mechanic　model　of　a　compressed　cell　under　puncture　is　established,　the　puncture　force　is　analyzed　by　using　ANSYS,　and　the　parameters　are　identified　by　MATLAB.　Secondly,　a　linear　optimization　model　is　proposed　for　obtaining　the　curve　of　injection　velocity　versus　time　by　defining　the　minimum　cell　deformation　as　the　objective　function.　Finally,　the　optimized　injection　puncture　velocity　trajectory　is　calculated　by　solving　the　proposed　optimization　model,　and　numerical　simulations　are　conducted　to　demonstrate　that　the　deformation　of　the　cell　during　the　puncture　process　is　reduced　with　optimized　puncture　velocity.　©　2019　IEEE.