The　paper　studies　the　effects　of　different　microstructures　on　the　hydrogen　resistance　properties.　Four　kinds　of　microstructures　were　obtained　by　using　four　kinds　of　heat　treatment　processes　for　45　steel　materials.　The　mechanical　properties　of　different　microstructure　materials　were　obtained　by　Vickers　hardness　test　and　internal　residual　stress　test.　The　relationships　between　hardness　and　depth　of　four　kinds　of　microstructures　were　obtained　by　stratified　microhardness　test.　Static　hydrogen　resistance　property　of　different　microstructures　was　obtained　by　using　the　stripping　method.　Results　show　that　the　H2　S　corrosion　layer　of　different　microstructures　is　basically　the　same,　and　the　depth　and　hardness　distribution　of　the　hydrogen　embrittlement　layer　are　quite　different.　More　pearlite,　lead　to　the　weakness　of　hydrogen　resistance　property;　quenched　and　tempered　sorbite　after　the　hydrogen　corrosion　is　better　than　ferrite　+　pearlite　structure,　martensite　has　a　good　resistance　to　hydrogen　permeability.　Finally,　the　relationships　between　microhardness　and　depth　of　the　hydrogen　embrittlement　layer　with　different　microstructures　were　fitted.　©　2018　Trans　Tech　Publications,　Switzerland.