Colorectal　cancer　(CRC)　is　the　third-leading　cause　of　cancer　mortality　worldwide.　HACE1　function　as　a　tumor-suppressor　gene　and　is　downregulated　in　several　kinds　of　cancers.　However,　the　distribution　and　clinical　significance　of　HACE1　in　CRC　is　still　not　clarified.　In　this　study,　we　found　that　the　HACE1　expression　is　greatly　downregulated　in　CRC　tissues　and　cell　lines.　Moreover,　the　HACE1　expression　was　significantly　associated　with　inhibition　of　CRC　cell　proliferation,　metastasis,　and　invasion.　HACE1　inhibited　epithelial-mesenchymal　transition　in　CRC　cells.　Furthermore,　we　found　that　HACE1　altered　the　protein　expression　of　the　Hippo　pathway　by　downregulation　of　YAP1.　HACE1　suppresses　the　invasive　ability　of　CRC　cells　by　negatively　regulating　the　YAP1　pathway.　Our　data　indicates　that　HACE1　directly　targets　YAP1　and　induces　downregulation　of　YAP1,　thereby　increasing　the　activity　of　the　Hippo　pathway.　In　summary,　these　findings　demonstrated　that　HACE1-YAP1　axis　had　an　important　part　in　the　CRC　development　and　progression.