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Author:

Sakhawat, Ali (Sakhawat, Ali.) | Ma, Ling (Ma, Ling.) | Muhammad, Tahir (Muhammad, Tahir.) | Khan, Aamir Ali (Khan, Aamir Ali.) | Chen, Xuechai (Chen, Xuechai.) | Huang, Yinghui (Huang, Yinghui.)

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Scopus SCIE PubMed

Abstract:

Breast cancer is the most prevalent malignancy in women, which remains untreatable once metastatic. The treatment of advanced breast cancer is restricted due to chemotherapy resistance. We previously investigated anti-cancer potential of a tumor selective oncolytic adenovirus along with cisplatin in three lung cancer cells; A549, H292, and H661, and found it very efficient. To our surprise, this virotherapy showed remarkable cytotoxicity to chemo-resistant cancer cells. Here, we extended our investigation by using two breast cancer cells and their resistant sublines to further validate CRAd's anti-resistance properties. Results of in vitro and in vivo analyses recapitulated the similar anti-tumor potential of CRAd. Based on the molecular analysis through qPCR and western blotting, we suggest upregulation of coxsackievirus-adenovirus receptor (CAR) as a selective vulnerability of chemotherapy-resistant tumors. CAR knockdown and overexpression experiments established its important involvement in the success of CRAd-induced tumor inhibition. Additionally, through transwell migration assay we demonstrate that CRAd might have anti-metastatic properties. Mechanistic analysis show that CRAd pre-treatment could reverse epithelial to mesenchymal transition in breast cancer cells, which needs further verification. These insights may prove to be a timely opportunity for the application of CRAd in recurrent drug-resistant cancers.

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Author Community:

  • [ 1 ] [Sakhawat, Ali]Beijing Univ Technol, Coll Life Sci & Bioengn, 100 Ping Le Yuan, Beijing 100124, Peoples R China
  • [ 2 ] [Ma, Ling]Beijing Univ Technol, Coll Life Sci & Bioengn, 100 Ping Le Yuan, Beijing 100124, Peoples R China
  • [ 3 ] [Muhammad, Tahir]Beijing Univ Technol, Coll Life Sci & Bioengn, 100 Ping Le Yuan, Beijing 100124, Peoples R China
  • [ 4 ] [Khan, Aamir Ali]Beijing Univ Technol, Coll Life Sci & Bioengn, 100 Ping Le Yuan, Beijing 100124, Peoples R China
  • [ 5 ] [Chen, Xuechai]Beijing Univ Technol, Coll Life Sci & Bioengn, 100 Ping Le Yuan, Beijing 100124, Peoples R China
  • [ 6 ] [Huang, Yinghui]Beijing Univ Technol, Coll Life Sci & Bioengn, 100 Ping Le Yuan, Beijing 100124, Peoples R China

Reprint Author's Address:

  • 黄映辉

    [Huang, Yinghui]Beijing Univ Technol, Coll Life Sci & Bioengn, 100 Ping Le Yuan, Beijing 100124, Peoples R China

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Source :

SCIENTIFIC REPORTS

ISSN: 2045-2322

Year: 2019

Volume: 9

4 . 6 0 0

JCR@2022

ESI Discipline: Multidisciplinary;

ESI HC Threshold:255

JCR Journal Grade:1

Cited Count:

WoS CC Cited Count: 15

SCOPUS Cited Count: 15

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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