Simple,　inexpensive,　and　rapid　diagnostic　tests　in　low-resource　settings　with　limited　laboratory　equipment　and　technical　expertise　are　instrumental　in　reducing　morbidity　and　mortality　from　epidemic　infectious　diseases.　We　developed　a　smartphone-based　fluorescent　lateral　flow　immunoassay　(LFIA)　platform　for　the　highly　sensitive　point-of-care　detection　of　Zika　virus　nonstructural　protein　1　(ZIKV　NS1).　An　attachment　was　designed　and　3D-printed　to　integrate　the　smartphone　with　external　optical　and　electrical　components,　enabling　the　miniaturization　of　the　instrument　and　reduction　in　cost　and　complexity.　Quantum　dot　microspheres　were　utilized　as　probes　in　fluorescent　LFIA　because　of　their　extremely　bright　fluorescence　signal.　This　approach　can　achieve　quantitative　point-of-care　detection　of　ZIKV　NS1　within　20　min.　Limits　of　detection　(LODs)　in　buffer　and　serum　were　0.045　and　0.15　ng　mL(-1),　respectively.　Despite　the　high　structural　similarity,　a　high-level　Dengue　virus　NS1　as　interferent　showed　limited　cross-reactivity.　Furthermore,　this　assay　was　successfully　applied　to　detecte　ZIKV　NS1　and　virions　spiked　in　complex　biological　samples,　indicating　its　practical　application　capability.　Given　its　low　cost,　compact　size,　and　excellent　analytical　performance,　the　proposed　smartphone-based　fluorescent　LFIA　platform　holds　considerable　potential　in　rapid　and　accurate　point-of-care　detection　of　ZIKV　NS1　and　provides　new　insight　into　the　design　and　application　of　molecular　diagnostic　methods　in　low-resource　settings.　(C)　2018　Published　by　Elsevier　B.V.