Alcohol　(EtOH)　dosage　and　exposure　time　can　affect　gene　expression.　However,　whether　there　exists　synergistic　effect　is　unknown.　Here,　we　analyzed　the　hDPSC　gene　microarray　dataset　GSE57255　downloaded　from　Gene　Expression　Omnibus　and　found　that　the　interaction　between　EtOH　dosage　and　exposure　time　on　gene　expression　are　statistically　significant　for　two　probes:　201917_s_at　near　gene　SLC25A36　and　217649_at　near　gene　ZFAND5.　GeneMania　showed　that　SLC25A36　and　ZFAND5　were　related　to　20　genes,　three　of　which　had　alcohol-related　functions.　WebGestalt　revealed　that　the　22　genes　were　enriched　in　10　KEGG　pathways,　four　of　which　are　related　to　alcoholic　diseases.　We　explored　the　possible　nonlinear　interaction　effect　and　got　172　gene　probes　with　significant　p-values.　However,　no　significantly　enriched　pathways　based　on　the　172　probes　were　detected.　Our　analyses　indicated　a　possible　molecular　mechanism　that　could　help　explain　why　alcohol　consumption　has　both　deleterious　and　beneficial　effects　on　human　health.