The　relationship　between　simultaneous　limbs　blood　pressure　differences　and　Ankle-Brachial　Index　(ABI)　is　controversial.　This　paper　aims　to　investigate　the　association　of　limbs　blood　pressures　differences　with　ABI　as　the　current　non-invasive　diagnosis　method　in　clinical　primary　care.　A　cross-sectional　study　was　performed　to　analysis　the　relationship　between　them.　The　results　showed　that　ABI　＜　0.9　was　independently　associated　with　inter-arm　(OR,　15.469;　CI,　(1.776-134.773);　P　=　0.013)　and　inter-ankle　(OR,　7.189;　CI,　(1.010-51.179);　P　=　0.049)　when　the　systolic　blood　pressure　difference　＞=　15mmHg.　Therefore,　the　simultaneous　measurement　of　four　limbs　blood　pressure　differences　can　provide　an　aid　for　the　non-invasive　detection　method　of　PAD　in　clinical　primary　care.