A　tidal　flow　constructed　wetland　(TFCW),　a　commonly　applied　system　to　clean　wastewater,　contains　a　substrate　to　assist　pollutants　removal,　while　the　choice　of　substrate　affects　the　formation　of　bacterial　biofilms.　Herein,　activated　alumina-TFCW　(A-TFCW)　with　hydraulic　load　of　1.35　m(3)/(m(2).d)　parallel　with　shale　ceramisite　(S-TFCW)　was　investigated　for　treating　domestic　wastewater,　aiming　to　enhance　simultaneous　long-term　removal　of　organics,　nitrogen　and　phosphorus.　A-TFCW　achieved　significantly　higher　COD,　NH4+-N,　TN　and　TP　removal　efficiency　than　S-TFCW,　with　the　removal　efficiency　of　85.9%　COD,　85.4%　NH4+-N,　72.8%　TN　and　96.4%　TP　respectively.　Denitrifying　bacteria　dominated　in　both　formed　biofilms,　with　higher　relative　abundance　of　nitrifying　bacteria　and　denitrifying　bacteria　in　A-TFCW.　These　results　demonstrated　that　AA　substrate　was　more　suitable　to　be　applied　in　enhancing　the　removal　performance　in　TFCW　for　the　treatment　of　domestic　wastewater.