Amblyopia　is　a　common　yet　hard-to-cure　disease　in　children　and　results　in　poor　or　blurred　vision.　Some　efforts　such　as　voxel-based　analysis,　cortical　thickness　analysis　have　been　tried　to　reveal　the　pathogenesis　of　amblyopia.　However,　few　studies　focused　on　alterations　of　the　functional　connectivity　(FC)　in　amblyopia.　In　this　study,　we　analyzed　the　abnormalities　of　amblyopia　patients　by　both　the　seed-based　FC　with　the　left/right　primary　visual　cortex　and　the　network　constructed　throughout　the　whole　brain.　Experiments　showed　the　following　results:　(1)As　for　the　seed-based　FC　analysis,　FC　between　superior　occipital　gyrus　and　the　primary　visual　cortex　was　found　to　significantly　decrease　in　both　sides.　The　abnormalities　were　also　found　in　lingual　gyrus.　The　results　may　reflect　functional　deficits　both　in　dorsal　stream　and　ventral　stream.　(2)Two　increased　functional　connectivities　and　64　decreased　functional　connectivities　were　found　in　the　whole　brain　network　analysis.　The　decreased　functional　connectivities　most　concentrate　in　the　temporal　cortex.　The　results　suggest　that　amblyopia　may　be　caused　by　the　deficits　in　the　visual　information　transmission.　©　2014　SPIE.