The　manganese-oxidizing　bacteria　were　embedded　in　polyvinyl　alcohol　that　was　cross-linked　with　boric　acid　and　then　processed　with　glutaraldehyde.　The　mechanical　stability　of　the　immobilized　bacteria　increased　with　the　concentration　of　polyvinyl　alcohol.　The　removal　rate　of　manganese　increased　with　the　concentration　of　bacteria　only　if　the　bacteria　concentration　was　lower　than　1:20(v/v).　Adding　activated　carbon　into　polyvinyl　alcohol　had　contributed　to　the　development　of　the　mass-transfer,　which　is　benefit　to　the　biological　bioactivity,　but　the　mechanical　stability　would　get　worse　if　too　many　activated　carbons　were　mixed　into　polyvinyl　alcohol.　The　simulated　filter　column　filled　with　the　immobilized　manganese-oxidizing　bacteria　was　cultivated　into　mature　stage　which　needed　about　30　days　and　the　bioactivity　of　the　immobilized　manganese-oxidizing　was　harmed　by　the　toxicity　of　the　cross　linking　agent　and　the　preparation　condition.　©　(2013)　Trans　Tech　Publications,　Switzerland.