A　series　of　halogenated　Phenstatin　analogs　were　designed　as　microtubule　destabilizing　agent　by　docking　study.　It　was　synthesized　within　three　steps　starting　from　2-chloro-5-iodobenzoic　acid　and　substituted　benzene.　All　the　products　were　characterized　by　H-1　NMR　and　C-13　NMR　spectral　analysis,　and　the　stereochemical　structure　was　also　confirmed　by　a　single　crystal　X-ray　diffraction　crystallographic　analysis.　The　microtubule　destabilizing　activities　were　evaluated　in　vitro　with　human　liver　cancer　Huh-7　cell　line　and　human　lung　cancer　A549　cell　line.　Some　of　the　HPAs　were　achieved　IC50　about　5.0M　against　human　liver　cancer　Huh-7　cells.　[GRAPHICS]　.