VC-co-VAc-OH　material　was　used　to　prepare　flat-sheet　membranes　on　the　non-woven　fabrics　via　phase　inversion　method.　And　the　membrane　preparation　conditions(polymer　content,　the　content　of　additive,　environmental　temperature,　relative　humidity,　evaporation　time,　coagulation　temperature,　coagulation　time,　immersing　time　of　non-woven　fabrics　in　solvent,　and　exposure　time　of　non-woven　fabrics　in　air)were　discussed　systemically　by　orthogonal　method.　Finally,　the　membrane　prepared　under　the　optimum　condition　showe　higher　pure　water　flux　(287.9　ml·cm-2·h-1)　and　good　retention　of　Bovine　serum　albumin　(BSA)　(87.4　%).　©　(2012)　Trans　Tech　Publications,　Switzerland.