As　we　all　knows　reducing　the　manufacturing　time　is　the　trend　of　precision　manufacturing,　and　the　precision　of　a　work-piece　is　very　important　for　manufacturing　industry.　High-speed　cutting　is　becoming　more　widely　used　and　the　high-speed　spindle　is　a　very　important　element,　whose　precision　may　affect　the　overall　performance　of　high-speed　cutting.　Firstly,　this　paper　introduces　the　two-point　method　origin　from　three-point　method　for　high-speed　spindle　rotation　error　motion　measurement,　and　ANSI/ASME　B5.54　which　is　the　testing　and　evaluation　standard　for　machine　tools.　Then　on　these　basis,　structure　of　the　spindle　dynamic　measurement　system　is　introduced　and　applied　in　details:　The　system　consists　of　a　displacement　measurement　unit　with　accuracy　up　to　0.5m,　within　600,000　rpm　for　various　spindles,　the　sampling　clock　modules,　data　acquisition　cards,　a　computer　and　data　processing　software.　The　analysis　of　the　experiments　shows　that　it　is　feasible　and　effective　to　use　the　proposed　methods,　does　not　need　any　reference　and　it　has　high　engineering　value　and　it　is　easy　to　set　up.　©　2011　IEEE.