Fungi　have　their　unique　advantages　in　capturing　and　degrading　hydrophobic　VOCs.　To　study　the　performance　of　fungi-based　bio-trickling　filters　(BTFs)　with　respect　to　the　degradation　of　toluene,　and　the　succession　process　of　the　fungal　colony　under　different　operating　conditions,　a　three-layer　BTF　packed　by　dominant　Fusarium　oxysporum　immobilized　with　ceramic　particles　were　set　up.　The　fungal　BTF　started　quickly　within　7　days　and　restarted　less　than　7　days　after　starvation;　its　average　RE　was　higher　than　92.5%　when　the　toluene　inlet　loading　rate　(ILR)　ranging　from　7.0　to　100.9　g　m(-3)　h(-1)　at　steady　state.　Moreover,　the　maximum　elimination　capacity　(EC)　of　98.1　g　m(-3)　h(-1)　was　obtained　at　a　toluene　ILR　of　100.3　g　m(-3)　h(-1).　The　microorganism　analysis　of　time　and　space　revealed　that　the　dominant　fungi　Fusariwn　were　replaced　by　Paramicrosporidium　saccamoebae　after　a　certain　evolutionary　period.　The　intermediate　layer　had　more　microbes　and　a　more　complex　community　than　the　other　two　layers,　and　was　more　suitable　for　the　survival　of　the　varieties　of　microbes.