The　chemical　surfactant　Tween　80　and　biosurfactant　rhamnolipid　were　added　to　the　solid-state　fermentation　processes　of　a　strain　of　actinomycete　Streptomyces　badius.　The　extracellular　amylase,　protease,　carboxymethyl　cellulose　enzyme　(CMCase)　and　xylanase　were　analyzed　during　the　fermentation　process.　In　addition,　the　pH　of　the　fermentation　substrate,　microbial　population　and　the　degradation　of　the　organic　matter　were　determined.　The　results　showed　that　Tween　80　increased　the　activities　of　amylase,　protease,　CMCase　and　xylanase　to　different　extents　while　rhamnolipid　only　had　an　obvious　stimulatory　effect　on　xylanase.　Neither　Tween　80　nor　rhamnolipid　had　an　obvious　effect　on　the　pH　of　the　fermentation　substrate.　As　a　result　of　the　improved　enzyme　activities,　the　degradation　rates　of　organic　matter　of　the　treatments　with　surfactants　were　higher　than　that　of　the　control.　The　results　indicated　that　Tween　80　is　a　common　stimulant　for　most　enzymes,　while　rhamnolipid　can　only　act　with　some　specific　enzyme,　such　as　xylanase.　©　2011　IEEE.