In　recent　years,　biological　purification　technology　has　been　widely　developed　in　the　process　of　iron　and　manganese　removal　from　groundwater.　The　cultivation　and　maturation　of　the　biological　filter　layer　are　key　for　biological　iron　and　manganese　removal　processes.　The　time　needed　for　maturation　varies　significantly　with　the　water　quality,　filter　and　filter　media　conditions　and　operation　parameters;　sometimes　it　takes　only　one　or　two　months,　sometime　more　than　half　a　year.　In　this　paper,　the　feasibility　of　adopting　an　intermittent　operation　for　the　cultivation　of　biofilter　was　investigated　with　productive　filters　in　a　groundwater　treatment　plant,　and　the　comparative　test　of　the　filter　column　was　conducted.　The　results　showed　that　the　intermittent　operation　had　little　effect　on　the　cultivation　of　the　biofilter　because　dissolved　oxygen　would　be　gradually　exhausted　during　the　filter-suspension　process,　making　the　filter　layer　anaerobic,　thus　possibly　inhibiting　the　growth　and　reproduction　of　IMOB　(Iron　and　Manganese　Oxidizing　Bacteria).　At　the　same　time,　the　test　shows　that　when　the　mature　biological　filter　needs　the　suspension　operation,　the　emptying　method　should　be　considered　to　avoid　the　destruction　of　the　biological　layer.