Objective:　Baseline　drift　of　pulse　waveform　caused　by　breathing　and　physical　movement　needs　to　be　removed　in　pulse　wave　detection　process.　This　paper　presents　a　removal　method　of　pulse　waveform　baseline　drift　using　cubic　spline　interpolation.　Methods:　Starting　points　of　pulse　waveform　are　selected　to　be　the　spline　interpolation　point.　And　cubic　spline　interpolation　according　to　the　starting　points　is　used　to　fit　the　baseline　drift.　Subsequently,　pulse　waveform　of　baseline　drift　removed　is　obtained　by　pulse　wave　data　minus　the　corresponding　baseline　drift　values.　Results:　Pulse　waveforms　after　removing　baseline　drift　are　stable　and　have　a　minor　relative　error.　Conclusion:　The　actual　measurement　results　show　that　the　method　can　inhibit　the　baseline　drift　effectively.　The　method　and　can　be　well　applied　in　real-time　detection　of　pulse　wave.　©　2010　IEEE.