hydrogen　sulfur　was　regarded　as　the　research　object　in　this　study.　Correlated　research　of　biological　treatment　of　hydrogen　sulfide　was　investigated.　Domestication　in　an　aeration　flask　was　employed　to　screen　out　the　optimal　bacteria　(Thiobacillus).　Na2S　was　used　as　the　inducer　in　the　domestication　period.　Gas-liquid　phase　joint　inoculation　method　could　start　up　biotrickling　filter　effectively.　The　effect　of　air　surface　velocity　(0.20-1.00　m3/h),　the　concentration　of　SO42　and　S2-　in　the　liquid,　empty　bed　residence　time　(EBRT)　(150-11s)　and　the　removal　capacity　were　investigated.　The　overall　start-up　period　was　only　2days　with　high　removal　efficiency　of　99%.　In　this　study,　biotrickling　filter　run　more　than　150　days　with　high　removal　performance.　The　optimal　empty　bed　residence　time　was　30　s　when　in　a　constant　inlet　loading　of　35.5gH2S/m3/h.　The　experiment　showed　that　domestication　in　aeration　flask　could　select　the　optimal　bacteria　to　degrade　H2S.　Gas-liquid　inoculation　method　could　shorten　the　start-up　period.　Furthermore,　the　biotrickling　filter　exhibited　a　high　performance　in　the　long　running.　©　2010　IEEE.