Subcellular localization of GFP-Tetherin fusion protein in COS-1 by confocal laser scanning microscope - 文章详情页

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EI Scopus

摘要：

tetherin　cDNA　was　amplified　from　total　RNA　prepared　from　HeLa　cells　by　RT-PCR,　then　cloned　into　the　eukaryotic　expression　vector　pEGFP-C3.　The　generated　pEGFP-Tetherin　construct　was　transfected　into　COS-1　to　determine　the　expression　and　the　subcellular　localization　of　GFP-Tetherin　fusion　protein.　Under　CLSM　the　localization　of　GFP-Tetherin　fusion　protein　distributed　outside　the　nucleus　and　co-localized　with　TGN　in　COS-1　cells.　©　2010　IEEE.

关键词：

Bioinformatics Biomedical engineering Cloning Computer viruses Diseases Lanthanum compounds Laser applications Polymerase chain reaction Proteins Viruses

作者机构：

[ 1 ] [Yang, Yishu]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
[ 2 ] [Lin, Xiaoyan]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
[ 3 ] [Sun, Xiaona]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
[ 4 ] [Wang, Xiaoli]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
[ 5 ] [Wu, Senren]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
[ 6 ] [Xiao, Xiangqian]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
[ 7 ] [Li, Zelin]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
[ 8 ] [Zeng, Yi]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China

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电子邮件地址：

yishu-y@bjut.edu.cn

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