Manganese-oxidizing　microorganisms　are　able　to　oxidize　soluble　Mn　2+　into　insoluble　Mn　oxides,　so　they　are　very　useful　in　treatment　of　Mn-contaminated　water.　A　strain　bacteria　Mn-103　was　isolated,　purified　from　manganese　removal　bio-filter　treated,　and　it　had　been　identified　to　belong　to　Bacillus　ce-1　by　the　MIDI　Sherlock　bacteria　identification　system.　The　strain　bacteria　has　the　manganese-oxidizing　ability　tested　by　the　LBB　method,　and　it　has　high　Mn2+　resistance,　the　Mn2+　concentration　in　the　culture　medium　that　less　than　30mmol/L　has　no　inhibitory　effect　for　Mn-103.　Study　on　the　growth　character,　the　results　showed　that　Mn-103　reached　period　of　logarithm　increasing　after　inoculation　6h,　then　it　entered　stabilization　period　after　inoculation　36h,　it　still　in　the　stabilization　period　until　60h,　and　it　can　induce　the　pH　of　surrounding　environment　increased.　This　bacterium　also　has　strong　Mn2+　biological　removal　ability,　after　inoculation　60h,　the　concentration　of　MnO2　can　reach　to　12　mg/L,　and　the　total　Mn　2+　biological　removal　amount　can　reach　about　20mg/L　in　the　culture　medium　that　the　original　total　Mn2+　concentration　is　about　27mg/L.　©2010　IEEE.