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The influences of pre-treatment temperature and time on bio-hydrogen production technology by anaerobic fermentation were studied in this paper, in order to provide basic data for quickening bio-hydrogen production technology. The results show that the best conditions are the temperature being 80°, the time being 10min and the PH value being 5.5, and the highest hydrogen production rate is 1.36mol/mol-glucose. In which conditions, acetic acid is the predominant fermentation metabolic product. ©2010 IEEE.
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