Nanocomposite　membranes　have　gained　growing　attention　in　various　application　fields,　especially　in　the　separation　and　purification　of　resource　substances.　However,　the　nanomaterials　and　their　membranes　fabrication　strategies　usually　suffer　from　complex　procedures,　high-energy　consumption　and　lack　of　universality.　Inspired　by　the　unique　self-polymerization　and　adhesion　characteristic　of　marine　mussels,　we　proposed　a　facile　and　versatile　approach　to　synthesize　zwitterionic　dopamine　nanoparticles　via　dopamine　induced　oxidative　polymerization　method,　followed　by　the　interfacial　cross-linking　on　porous　supporting　layer　to　fabricate　thin-film　nanocomposite　(TFN)　membranes.　The　influences　of　nanoparticle　composition,　particle　size　and　loading　content　on　the　membrane　structure　and　performance　were　studied　to　optimize　the　fabrication　conditions.　The　resultant　TFN　membrane　exhibited　high　water　permeance　(98.0　L　m(-2)　h(-1)　MPa-1)　and　good　selectivity　for　di-/mono-valent　salts　(S-Na2SO4/NaCl　=　similar　to　4.50,　S-MgSO4/NaCl　=　similar　to　4.49).　Furthermore,　by　utilizing　the　protein,　polysaccharide,　and　humic　acid　as　model　foulants,　the　zwitterionic　dopamine　nanoparticles　based　TFN　membranes　have　been　demonstrated　with　excellent　fouling　resistance　and　cleaning　recovery　property　in　separation　applications.