Culture　medium＇s　entrying　and　sterilizing　process　has　the　characteristics　of　nonlinearity,　long　time　lag,　big　inertia,　with　lots　of　influencing　factors　and　control　complexity.　So　it　is　very　difficult　for　the　conventional　PID　controller　to　control　the　process.　In　most　ferment　factories,　the　process　was　controlled　manually.　It　has　the　disadvantages　of　high　labor　intensity　and　low　accuracy　of　temperature　control.　Therefore,　the　paper　applies　feed-forward　control　and　self-tuning　fuzzy-PID　control　to　the　entrying　and　sterilizing　process,　and　a　satisfying　automatic　control　has　realized　in　a　pharmaceutical　factory＇s　culture　medium＇s　entrying　and　sterilizing　process.　The　results　of　the　control　show　that　the　controller　built　in　the　paper　can　improve　control　accuracy　and　shorten　the　system　response　time,　avoid　the　occurrence　of　bacteria　contaminating　and　increase　fermentation　unit.　The　temperature　accuracy　in　the　balance　state　is　±　0.5°C.　©　2006　IEEE.