A　high-frequency　and　micro-amplitude　vibration　based　micromanipulator　is　proposed,　which　is　composed　of　a　micro-motion　parallel　mechanism　having　three　pure　translational　degrees　of　freedom　and　a　micro-vibration　unit　having　one　degree　of　freedom.　The　micro-vibration　unit　is　fixed　on　movable　platform　of　the　parallel　mechanism,　and　an　glass　operational　needle　is　connected　with　the　micro-vibration　unit.　The　needle　can　perform　micro　operational　tasks　while　the　movable　platform　of　parallel　mechanism　moves　in　a　large　region　and　the　unit　vibrates　with　high　frequency　and　micro　amplitude.　The　kinematic　and　structural　parameters　of　the　micro-motion　mechanism　and　vibration　unit　are　designed,　the　PZT　actuators　are　chosen,　and　a　micromanipulator　prototype　is　fabricated.　An　experiment　system　is　set　up　in　which　the　micromanipulator　is　adopted　as　a　main　module.　The　performance　of　micromanipulator　is　validated　through　two　initial　operational　experiments,　one　is　the　vibrational　injection　of　cow　oocyte,　another　is　the　windowing　of　cow　oocyte　membrane,　the　results　of　experiments　show　that　the　micromanipulator　can　complete　these　operations　successfully.　©　2006　IEEE.