To　study　the　impact　of　printing　process　on　the　cell　viability,　two　types　of　human　living　cells,　human　peripheral　blood　mononuclear　cells　(hPBMCs,　10-15　μm　in　diameter)　and　human　bronchial　epithelial　cells　(hBECs,　50-70　μm　in　diameter),　were　printed　at　several　jetting　frequencies　from　10　Hz　to　70　Hz.　The　printing　device　was　a　home-made　pneumatic　drop-on-demand　generator　with　a　diameter　nozzle,　and　producing　micro-droplet　with　size　of　180-200　μm.　Non-ejected　cell　suspension　was　taken　as　control　group,　while　in　experimental　groups　about　6　000　drops　of　cell　suspension　were　delivered　to　sample　tube　containing　phosphate　buffered　saline　(PBS)　for　each　jetting　frequency.　Then　7-AAD　dye　and　cytometry　methodwere　utilized　to　evaluate　the　cell　viability.　The　relative　viabilities　of　hPBMCs　and　hBECs　are　0.991±0.009　and　0.996±0.014,　respectively.　The　experimental　analysis　shows　that　the　jetting　frequency　has　no　significant　effect　on　cell　viability,and　the　pneumatic　printing　device　can　reach　high　cell　viability　that　may　result　from　the　low　viscosity　of　printing　cell-load　suspension　and　low　shear　stress　during　the　cell　printing　process.　The　pneumatic　printing　can　be　successfully　applied　in　the　fields　of　bioprinting.　©　2019,　Editorial　Department　of　Journal　of　Beijing　University　of　Technology.　All　right　reserved.