Microscopic　stereovision　systems　with　a　stereo　light　microscope　have　been　used　for　quantized　observations　and　measurements　in　microscopy　fields.　These　applications　depend　on　a　quantization　mathematical　relationship　between　the　object　space　and　the　image　space.　In　this　article,　a　novel,　practical　space　quantization　method　was　proposed.　It　was　derived　from　two　defined　base　cell　matrices.　First,　it　captured　a　series　of　stereo　images　of　markers　moving　in　various　directions　through　the　object　space.　The　world　and　image　coordinates　of　markers　were　acquired,　stored　and　used　to　create　the　two　base　cell　matrices.　Next,　a　space　quantization　relationship　between　the　object　space　and　the　image　space　was　derived　from　analysis　of　the　two　base　cell　matrices.　Finally,　if　two　matching　points　located　in　the　image　space　were　given,　the　world　coordinates　of　their　object　point　could　be　calculated　by　the　above　space　quantization　relationship.　The　results　show　that　this　method　of　quantizing　the　two　spaces　could　achieve　a　positioning　precision　of　approximately　+/-　5　mu　m　in　the　horizontal　direction　and　approximately　+/-　10　mu　m　in　the　vertical　direction.　It　has　a　very　simple　expression,　but　it　is　more　practical　and　efficient　than　other　traditional　projection　methods.　In　addition,　it　has　a　better　performance　and　can　be　widely　used　in　several　microscopy　fields,　such　as　microscopic　shape　reconstruction,　micromanipulation,　microassembly,　and　microinjection.