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In order to achieve efficient removal of nitrogen from high ammonia nitrogen wastewater at room temperature, this study selected three gas water ratio conditions, 8:1, 12:1 and 15:1 respectively. It investigated the stable operation performance of partial nitrification-ANAMMOX integrated autotrophic nitrogen removal process of biological aerated filter (BAF) at room temperature. The results showed that gas water ratio (GWR) at the optimal operation time was 15:1under the condition of inlet ammonia nitrogen (NH4 +-N) concentration of 400mg/L and reflux ratio of 1:1, ammonia nitrogen removal rate (ARE) was over 90%,total nitrogen (TN) removal load was 1.1kgN/(m3•d), and TN removal rate could reach 83%. When the GWR is 15:1,the DO is controlled around 2.41mg/L and 4.22mg/L, the amount of NH4 +-N converted into nitrite (NO2 --N) in water increased, and the ANAMMOX activity is enhanced. The real-time fluorescence quantitative PCR (QPCR) analysis of functional strains on the biofilm showed that when the GWR was 15:1, both ANAMMOX and AOB had the highest abundance, more than 1012 copies/g dry sludge. Therefore, integrated nitrogen removal has the best effect. At the same time, studies have shown that the ANAMMOX reaction is strengthened after increasing the gas water ratio and N2O is not generated in the ANAMMOX process. When the GWR is 15:1, the total release amount of N2O is the smallest, and the release factor is 0.0012. © 2019, Editorial Board of China Environmental Science. All right reserved.
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