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In order to assess the feasibility of restoring sewage partial nitritation/anammox (PN/A) process using free nitrous acid (FNA) to treat flocculent sludge, the effect of different concentrations of FNA on the activities of ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) was studied. Besides, the variations of nutrient removal performance in the SBR reactor by twice FNA treatment were investigated. The results showed that NOB activity could be inhibited when the flocculent sludge treated by FNA of 0.45mgHNO2-N/L and the nitrite accumulation rate (NAR) reached 88.8%, but NOB activity recovered from the 8th day. While NOB activity could be significantly inhibited when the flocculent sludge treated by FNA of 1.35mgHNO2-N/L, and the NAR reached 89.1%. Meanwhile the activities of AOB was also inhibited, and NH4 +-N conversion rate decreased to 6.8%. By enhancing the ratio of aerobic time to anoxic time (t好/t缺: from 0.4~2.7) and increasing dissolved oxygen (DO: from 0.2~1.5mg/L), the AOB activity recovered, and NH4 +-N conversion rate reached 77.8%. But the activities of NOB was not recovered within 150days, and the NAR achieved 98.1%. With the stable achievement of nitritation, the nitrogen removal performance of the system gradually restored, the average effluent total inorganic nitrogen (TIN) was 8.2mg/L, and the average TIN efficiency was 84.1%. Therefore, the strategy by first treating flocculent sludge with higher FNA to inhibite both AOB and NOB, then increasing t好/t缺 and DO to restore AOB activity, could achieve the restoration of PN/A process. © 2019, Editorial Board of China Environmental Science. All right reserved.
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