Graphene　oxide　(GO)　was　used　to　enhance　denitrification　performance　of　anammox　bacteria.　Batch　experiments　were　executed　to　explore　effect　of　GO　on　anammox　bacteria.　The　results　showed　anammox　bacteria　had　the　best　nitrogen　removal　performance　when　the　concentration　of　GO　was　0.15g/L,　and　the　total　nitrogen　removal　rate　was　18.6%　higher　than　blank　group.　When　GO　concentration　reached　0.2g/L,　anammox　bacteria　activity　was　inhibited,　and　the　total　nitrogen　removal　rate　was　reduced　by　26.0%　compare　with　the　blank　group.　A　long-term　experiment　was　operated　to　ident　the　effect　of　GO　on　anammox　bacteria　by　set　control　group.　The　results　showed　the　average　total　nitrogen　removal　rate　of　the　R2　reactor　with　GO　was　85.3%,　83.2%　and　81.1%　and　80.8%,　respectively,　which　was　higher　than　the　R1reactor　without　GO.　The　kinetics　characteristics　of　nitrogen　removal　in　a　single　cycle　of　the　R2　reactor　were　evaluated.　The　modified　Boltzmann　and　Gompertz　model　were　found　to　be　the　appropriate　models　to　describe　the　denitrification　performance　of　the　R2　reactor　in　a　single　cycle.　Moreover,　the　formula　to　predict　the　total　nitrogen　effluent　concentration　and　removal　rate　at　any　time　in　the　cycle　was　obtained.　©　2019,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.