Hydroxyapatite　(HA)　coatings　are　being　widely　used　in　biomedical　applications　owing　to　their　excellent　biocompatibility　and　osteoconductivity.　Recent　studies　have　demonstrated　that　the　crystallographic　texture　plays　an　important　role　in　improving　the　chemical　stability　and　mechanical　properties　of　HA　coatings.　In　this　study,　optimized　APS　parameter　was　selected　to　deposit　HA　coatings　with　strong　(002)　crystallographic　texture,　high　phase　purity　and　enhanced　melting　state.　Cross-sectional　SEM　images　show　uniformly　distributed　columnar　grains　perpendicular　to　the　coating　surface.　To　study　the　formation　conditions　of　columnar　grains,　coatings　with　distinct　microstructure　were　deposited　with　different　spray　parameters.　Moreover,　HA　coatings　were　deposited　on　substrates　with　varying　temperatures　such　as　25,　300　and　600　degrees　C　at　a　long　stand-off　distance　to　evaluate　the　role　of　the　substrate　temperature　in　the　formation　of　columnar　grains.　The　results　indicate　that　completely　molten　in-flight　particles　and　slow　cooling　rate　are　necessary　conditions　to　form　a　strong　crystallographic　texture.　The　present　study　suggests　that　the　crystalline　structure　of　HA　coatings　deposited　and　formed　by　APS　could　be　well　controlled　by　modifying　spray　parameters　and　substrate　temperature.