Objective:　To　study　the　effect　of　anti-HIV　III　B　virus　with　extractions　from　Juglans　regia,　so　as　to　searching　for　the　new　and　efficacious　leading　compound　of　AIDS　therapy.　Method:　Phytochemical　and　chromatographical　techniques　were　used　to　isolate　compounds　from　J.　regia;MT4　cells　and　HIV　III　B　virus　were　used　to　study　the　effect　of　anti-HIV　activity　in　vitro.　BIACORE®3000　molecule　coupled　equipment　were　used　for　the　target　research　also.　Result:　Two　extractions　(B&E)　were　isolated　from　J.　regia　which　possess　the　effect　of　anti-HIV　activity.　Targets　study　found　that　extraction　B　could　affected　on　HIV-1　gp-41　fusing　protein　and　extraction　E　could　affected　on　HIV-1　integrase　respectively.　Conclusion:　J.　regia　is　a　traditional　Chinese　medicine　with　active　anti-HIV　activity　and　worth　to　be　studied　further.