OBJECTIVE:　To　construct　recombinant　adenovirus　containing　codon-modified　HPV16L1　gene,　and　evaluate　systemic　and　mucosal　immunological　responses　induced　after　immunization　with　the　recombinant　virus.　METHODS:　The　recombinant　adenovirus　rAd-mod.HPV16L1　was　constructed　by　Admax　kit.　The　C57　BL/6　mice　were　immunized　by　purified　rAd-mod.HPV16L1　through　different　inoculation　routes.　The　immunological　effect　was　evaluated　by　testing　the　specific　neutralizing　antibodies　in　sera　and　vaginal　secretions　of　immunized　mice　through　indirect　ELISA　and　neutralization　assay　based　HPV　pseudovirus.　RESULTS:　The　result　showed　that　intramuscular　immunization　could　induce　good　systemic　immunity,　but　the　mucosal　immunity　was　too　weak,　and　immunization　via　intranasal　route　could　induce　satisfactory　immunity　both　in　sera　and　vaginal　secretions,　while　intravaginal　immunization　failed　to　induce　any　specific　immunological　responses　either　in　sera　or　vaginal　secretions.　CONCLUSION:　The　recombinant　adenovirus　containing　codon-　modified　HPV16L1　gene　was　successfully　constructed.　Immunization　through　intranasal　route　could　induce　satisfactory　immunity　both　in　sera　and　vaginal　secretions,　while　intramuscular　immunization　could　only　induce　high　titer　of　neutralizing　antibodies　in　sera.