The　partial　denitrification　(NO　3　-　-N→NO　2　-　-N),　a　promising　way　for　nitrate　wastewater　treatment,　could　combine　with　ANAMMOX　technology　to　achieve　nitrogen　removal　with　low　concentration　of　carbon　source　and　poor　energy.　Three　different　domestication　pattern,　R1　(SBR　under　anoxic　condition),　R2　(SBR　under　anoxia-aerobic　alternating　condition)　and　R3　(SBR　under　anoxic　condition　with　low-intensity　aeration),　were　conducted　using　glucose　as　electron　donor　to　find　out　the　rule　of　nitrite　accumulation　in　denitrification　process.　These　three　reactors　achieved　steady　nitrite　accumulation　after　120-days　run,　and　the　sludge　were　taken,　respectively,　to　explore　the　denitrification　pattern　of　these　three　acclimatizing　ways.　The　findings　revealed　that,　compared　with　R1,　the　R2　and　R3　sludge　could　better　achieve　partial　denitrification　due　to　the　involving　of　dissolved　oxygen.　And　R3　was　the　best　way.　The　microbial　community　structure　of　R1,　R2　and　R3　sludges　on　the　109th　day　were　analyzed　and　compared　by　high　throughput　sequencing.　The　results　showed　that　the　dominant　bacteria　were　Candidatus　Saccharibacteria　in　both　R2　and　R3　reactor,　which　were　with　the　participation　of　dissolved　oxygen,　and　the　relative　abundance　was　45.44%　and　34.　96%,　respectively.　This　was　the　first　time　that　the　Candidatus　Saccharibacteriawas　reported　as　the　dominant　bacteria　in　the　denitrifying　sludge.　Besides,　the　microbial　diversity　of　R1reactor　was　much　larger　than　that　of　R2　and　R3,　which　indicated　that　the　denitrifying　bacteria　in　R2　and　R3　reactors　were　more　exclusive.　The　batch　experiments　showed　that　the　initial　pH　had　a　significant　effect　on　the　accumulation　of　nitrite.　The　higher　the　pH　indicated　the　higher　accumulation　rate　of　nitrite.　©　2018,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.