Using　polyethylene　glycol　diacrylate　as　carrier　materials,　respectively　with　N,　N,　N,　‘N’-tetramethylethylenediamine　(TEMED)　as　promoter　and　potassium　persulfate　(KPS)　as　initiator　to　immobilize　anaerobic　ammonium　oxidizing　bacteria.　Orthogonal　experiments　were　used　to　optimize　immobilization　conditions　of　anaerobic　ammonium　oxidation　bacteria:　10%　PEGDA　monomer,　0.25%　KPS,　0.5%　TEMED,　the　optimum　operation　condition　was　to　control　the　polymerization　temperature　at　20℃,　polymerization　time　was　about　5min,　The　ratio　of　bacteria　to　gum　was　1:1.　The　continuous　flow　experiment　of　immobilized　pellets　showed　that　after　a　short　period　of　activity　recovery,　the　nitrogen　removal　effect　was　continuously　improved,and　it　had　a　certain　impact　resistance　to　the　increase　of　hydraulic　load.　The　scanning　electron　microscopy　(SEM)　showed　that　the　immobilization　materials　have　good　biocompatibility　and　good　mass　transfer　performance.　High-throughput　sequencing　showed　that　after　a　period　of　time　of　stable　operation,　the　microbial　diversity　in　the　pellets　decreased　slightly.　And　the　Candidatus　Kuenenia　that　belongs　to　anaerobic　ammonium　oxidizing　bacteria　which　accounted　for　6.58%　of　the　total　microbial　system　was　up　to　9.8%,　the　changes　in　microbial　population　showed　that　the　performance　of　anaerobic　ammonium　oxidation　could　be　improved　greatly　after　immobilization.　©　2018,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.