To　investigate　the　feasibility　of　bio-oil　generated　from　waste　cooking　oil　as　anti-aging　agent　for　the　petroleum　asphalt,　the　90#　control　asphalt　binder　and　the　bio-oil　produced　by　waste　cooking　oil　were　selected.　The　bio-oil　modified　asphalt　binders　were　obtained　according　to　the　high　speed　blend　tests.　The　road　performance　of　the　control　asphalt　binder　and　bio-oil　modified　asphalt　binders　were　evaluated　using　the　dynamic　sheer　rheometer　and　bending　beam　rheometer.　Results　show　that　with　the　increase　of　the　bio-oil　content,　bio-oil　modified　binders　present　better　fatigue　performance　and　low　temperature　performance　than　the　control　asphalt　binder,　while　the　bio-oil　modified　binders　show　a　worse　rutting　resistance　than　the　control　asphalt　binder.　©　2018,　Editorial　Department　of　Journal　of　Beijing　University　of　Technology.　All　right　reserved.