Phosphorylated　peptides　are　important　for　understanding　the　processes　of　biological　regulation.　However,　the　detection　of　phosphopeptides　remains　a　challenge　because　of　their　low　abundance　and　the　suppression　by　non-phosphopeptides.　Here,　we　report　a　strategy　for　the　facile　and　rapid　fabrication　of　TiO2-nanoparticle-packed　microchannel-array　glass　microchips　(TMA-microchips)　for　in-tube　solid-phase　microextraction　(IT-SPME)　using　a　plasma-assisted　method　for　the　precise　alignment　of　the　microstructure.　This　proposed　strategy　was　applied　to　the　selective　enrichment　of　phosphopeptides　from　a　protein　digestion　mixture,　demonstrating　the　high　capacity　and　selectivity　of　the　SPME　microchips.　An　important　feature　of　this　array　design　is　that　it　fully　exploits　the　advantage　of　nanoparticles　for　improving　extraction　capacity　and　simultaneously　provides　an　effective　way　to　reduce　the　pressure　for　driving　solutions;　thus,　it　paves　the　way　for　future　methods　that　simultaneously　take　advantage　of　nanomaterials　and　microchips.　(c)　2018　Elsevier　B.V.　All　rights　reserved.