Although　pulsatile　ECMO,　as　novel　kinds　of　ECMO,　has　been　attracted　more　and　more　attention,　the　differences　of　the　hemodynamic　effects　of　the　pulsatile　ECMO　on　the　aorta,　the　cerebral　perfusion,　and　left　ventricular　work　were　still　under-investigated.　The　aim　of　this　study　was　to　clarify　the　hemodynamic　differences　of　the　cardiovascular　system　between　the　pulsatile　and　non-pulsatile　VA　ECMO.　In　this　study,　three　ECMO　support　modes,　named　as　＂constant　flow　mode＂,　＂co-pulse　mode＂　and　＂counter　pulse　mode＂,　were　designed.　The　computational　fluid　dynamics　(CFD)　study　was　carried　out.　The　distribution　of　the　oxygenated　blood,　the　blood　velocity　vector,　the　oscillatory　shear　index　(OSI),　the　relative　residence　time　(RRT),　the　left　ventricular　external　work　(EW),　the　equivalent　left　ventricular　afterload　(EAL)　and　the　energy　loss　of　cardiovascular　system　(EL)　were　calculated　to　compare　the　hemodynamic　differences.　The　simulation　results　demonstrate　that　the　oxygenated　blood　under　co-pulse　mode　was　easier　to　perfuse　into　the　three　braches　vessels　than　that　under　both　other　modes.　In　addition,　the　ECMO　under　counter　pulse　mode　could　also　achieve　lowest　RRT　(constant　flow　mode　220　vs.　co-pulse　mode　132　vs.　counter　pulse　mode　93).　Similarly,　the　ECMO　under　counter　pulse　mode　could　significantly　reduce　the　left　ventricular　external　work　(co-pulse　mode　1.51　w　vs.　constant　flow　mode　1.44　w　and　counter　pulse　mode　1.30　w),　left　ventricular　afterload　(constant　flow　mode　1.03　mmHg.s/ml　vs.　co-pulse　mode　1.67　mmHg.s/ml　vs.　counter　pulse　mode　0.82　mmHg.s/ml)　and　energy　loss　of　arterial　system　(constant　flow　mode　0.18　w　vs.　co-pulse　mode　0.50　w　vs.　counter　pulse　mode　0.16　w).　In　short,　the　ECMO　under　counter　pulse　mode　could　have　advantages　to　the　left　ventricular　unloading.　In　contrast,　the　ECMO　under　co-pulse　mode　has　more　benefit　to　cerebral　oxygen　perfusion.