Liquid　biopsies　using　body　fluids　have　gained　much　attention　in　recent　years　due　to　their　multiple　advantages　in　clinical　diagnosis,　such　as　less/non-invasive　collection,　suitability　for　longitudinal　disease　monitoring,　and　better　representation　of　tumor　heterogeneity.　As　an　attractive　choice　for　liquid　biopsy,　urine　proteins　and　their　post　translational　modifications　(PTMs)　have　the　potential　to　offer　significant　insights　into　physiological　variations　and　pathological　changes　in　the　human　body.　However,　due　to　the　intrinsically　large　variability　of　urine　proteins　and　their　PTMs　among　different　individuals,　there　is　a　high　demand　for　strategies　for　high-throughput　analysis　of　a　large　number　of　samples　to　obtain　a　comprehensive　view　and　a　reliable　reference　interval　of　the　urine　proteome.　In　this　work,　we　proposed　a　new　urine　phosphoproteome　sample　processing　strategy　that　combines　fast　protein　extraction,　efficient　multiple　immobilized-proteases　digestion,　and　tandemly　connected　centrifugal　tips　device-based　facile　phosphopeptide　enrichment　&　fractionation.　This　strategy　is　capable　of　paralleled　sample　processing　with　an　approximate　five-fold　reduction　in　processing　time　and　is　therefore　particularly　suitable　for　handling　a　large　number　of　urine　samples.　Totally,　we　identified　4196　phosphosites　in　human　urine　proteins　by　mass　spectrometry　in　replicated　tests,　a　number　which　is　dozens　of　times　larger　than　those　previously　reported.　Therefore,　this　strategy　may　have　great　potential　in　urine-based　phosphoprotein　biomarker　screening　and　drug　response　studies.