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Author:

Zhang, Mingzi (Zhang, Mingzi.) | Liu, Shu (Liu, Shu.) | Guan, Enling (Guan, Enling.) | Liu, Hao (Liu, Hao.) | Dong, Xinhang (Dong, Xinhang.) | Hao, Yan (Hao, Yan.) | Zhang, Xin (Zhang, Xin.) | Zhao, Pengxiang (Zhao, Pengxiang.) | Liu, Xuehua (Liu, Xuehua.) | Pan, Shuyi (Pan, Shuyi.) | Wang, Youbin (Wang, Youbin.) | Wang, Xiaojun (Wang, Xiaojun.) | Liu, Yifang (Liu, Yifang.)

Indexed by:

Scopus SCIE PubMed

Abstract:

Background: Hyperbaric oxygen therapy (HBOT) has been widely used in the clinical setting. In this study, HBOT therapy was evaluated for its ability to ameliorate the epithelial-to-mesenchymal transition (EMT) phenomenon in keloid tissue. Methods: Keloid patients were randomly divided into two groups: keloid patients (K group, 9 patients) and keloid patients receiving HBOT (O group, 9 patients). A third group with normal skin (S group, 9 patients) was established for control. Before HBOT and surgery, a laser Doppler flowmeter was used to measure the keloid blood supply of patients in the O group. Hematoxylin and eosin (H&E) staining was used to observe morphology. E-cadherin, ZO-1, vimentin, fibronectin, vascular endothelial growth factor (VEGF), and hypoxia inducible factor (HIF)-1 alpha were measured by immunofluorescence staining and Western blot analysis. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the mRNA expression level of these factors as well. Results: In the O group, keloid blood perfusion was significantly reduced after patients received HBOT. Compared with the K group, lower expression levels of vimentin, vibronectin, VEGF, and HIF-1 alpha were observed in the O group, whereas the expression of E-cadherin and ZO-1 was significantly higher. The mRNA expression of E-cadherin and ZO-1 was also increased after HBOT. Conclusions: The expression levels of factors related to the EMT phenomenon were significantly reversed in keloid patients after they received HBOT, indicating that HBOT may be an effective therapy against the EMT phenomenon in keloid patients.

Keyword:

keloid epithelial-to-mesenchymal transition hyperbaric oxygen therapy

Author Community:

  • [ 1 ] [Zhang, Mingzi]North China Univ Sci & Technol, Peking Union Med Coll Hosp, Dept Plast Surg, Beijing, Peoples R China
  • [ 2 ] [Liu, Hao]North China Univ Sci & Technol, Peking Union Med Coll Hosp, Dept Plast Surg, Beijing, Peoples R China
  • [ 3 ] [Dong, Xinhang]North China Univ Sci & Technol, Peking Union Med Coll Hosp, Dept Plast Surg, Beijing, Peoples R China
  • [ 4 ] [Hao, Yan]North China Univ Sci & Technol, Peking Union Med Coll Hosp, Dept Plast Surg, Beijing, Peoples R China
  • [ 5 ] [Wang, Youbin]North China Univ Sci & Technol, Peking Union Med Coll Hosp, Dept Plast Surg, Beijing, Peoples R China
  • [ 6 ] [Wang, Xiaojun]North China Univ Sci & Technol, Peking Union Med Coll Hosp, Dept Plast Surg, Beijing, Peoples R China
  • [ 7 ] [Liu, Shu]North China Univ Sci & Technol, Dept Plast Surg, China Meitan Gen Hosp, Beijing, Peoples R China
  • [ 8 ] [Guan, Enling]Qingdao Huangdao Dist Hosp Tradit Chinese Med, Dept Ear Nose Throat, Qingdao, Shandong, Peoples R China
  • [ 9 ] [Zhang, Xin]Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing, Peoples R China
  • [ 10 ] [Zhao, Pengxiang]Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing, Peoples R China
  • [ 11 ] [Liu, Xuehua]Beijing Chao Yang Hosp, Dept Hyperbar Oxygen, Beijing, Peoples R China
  • [ 12 ] [Pan, Shuyi]Navy Gen Hosp, Dept Hyperbar Oxygen, Beijing, Peoples R China
  • [ 13 ] [Liu, Yifang]Beijing Vocat Coll Agr, Int Educ Coll, Beijing, Peoples R China

Reprint Author's Address:

  • [Wang, Youbin]Peking Union Med Coll Hosp, Dept Plast Surg, Shuaifuyuan 1, Beijing 100730, Peoples R China

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Source :

MEDICINE

ISSN: 0025-7974

Year: 2018

Issue: 29

Volume: 97

1 . 6 0 0

JCR@2022

ESI Discipline: CLINICAL MEDICINE;

ESI HC Threshold:167

Cited Count:

WoS CC Cited Count: 27

SCOPUS Cited Count: 25

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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