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Under room temperature, for learning the effects of aeration and temperature on start-up of shortcut nitrification, secondary effluent which is got from domestic wastewater after A/O process is treated in a sequencing batch biofilm reactor(SBBR). In the initial experiment, the SBBR is filmed naturally, and then nitrite oxidizing bacteria(NOB)are restrained by keeping high concentration of FA and low concentration of DO in order to make ammonia oxidizing bacteria(AOB)be the dominant bacterium. The tested results prove that the nitration biofilm in SBBR is cultivated and enriched successfully in 12 days, it is fast to get film. On the assumption that shortcut nitrification is not impacted, NOB could be restrained effectively in low concentration of DO, it is favorable for AOB to be the superior bacterium in the reactor and ammonia nitrogen transformation of influent is not impacted. On the condition of low concentration of ammonia nitrogen, lower temperature restrains the activity of AOB, and nitrite accumulation rate could not increase steadily by shortening the aeration time.
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