The　cultivation　of　the　denitrifying　phosphorus　removal　bacteria　(DPB)　in　biological　nutrient　removal　system　will　directly　affect　the　removal　efficiency　of　the　nutrients　in　wastewater　such　as　nitrogen　and　phosphorus,　and　the　stability　of　the　process.　For　this　reason,　a　new　BNR　process,　which　is　called　double-sludge　denitrifying-nitrogen　and　phosphorus　removal　process,　was　designed.　In　order　to　create　a　good　anaerobic/anoxic　alternating　environment　for　DPB,　gradual　transition　of　training　methods　were　used　to　make　DPB　natural　selection,　and　then　finally,　screened　the　dominated　DPB　bacteria.　The　results　indicated　that　the　anaerobic/aerobic　(A/O)　operation　mode　can　induce　induction　of　PAOs　rapidly　by　15　days　intermittent　aeration.　The　proportion　of　DPB/PAOs　in　the　reactor　was　aggrandized　in　the　second　phase　(about　25　days　inducement)　by　reducing　the　aeration　time　in　aerobic　phase　gradually　and　adding　nitrate　in　anoxic　phase,　which　is　called　anaerobic/aerobic/anoxic　(A/O/A)　mode;　finally,　the　strictly　alternating　environmental　anaerobic/anoxic　conditions　for　DPB　were　used　to　induce　and　enrich　the　target　DPB　bacteria　for　19　days　by　a　gradual　transition　of　enrichment　culture.　The　successful　domestication　and　cultivation　of　DPB　provides　a　new　method　to　remove　nitrogen　and　phosphorus　efficiently　and　steadily　in　municipal　wastewater.