A　facile　one-step　method　to　the　immobilization　of　the　combination　of　glucose　oxidase(GOD)　and　catalase(CAT)　in　mesostructured　TiO2　was　proposed.　The　results　obtained　by　transmission　electron　microspectroscopy　and　nitrogen　adsorption-desorption　analysis　clearly　show　that　the　TiO2　mediated　by　the　combination　of　GOD　and　CAT(CGC)　has　a　large　surface　area　and　a　narrow　pore-size　distribution.　The　CGC　immobilized　on　mesostructured　TiO2　exhibits　direct　electrochemistry　and　good　electrocatalytic　performance　without　any　electron　mediator.